Table 1.
Summary of iPSCs generated from domestic animals
| Species | Cell sources | Basal media | Media supplements | Feeder layers | In vitro differentiation | In vivo differentiation | Reprogram method | Karyotype / Results | References |
|---|---|---|---|---|---|---|---|---|---|
| Dog | Embryonic fibroblasts | Primate ES medium (ReproCELL, Japan) | bFGF, hLIF, VPA, PD0325901, CHIR99021, A83-01 | MEFs | None | None | Retrovirus (Canine OKSM) | None / None | [44] |
| Testicular fibroblasts (7 mos) | DMEM/F12, 15% KSR | bFGF, hLIF | MEFs | EB | None | Lentivirus (Human OKSM) | G-banding / Normal | [94] | |
| Adipose stromal cells, Skin fibroblasts (1 yr) | Knockout DMEM 20% ES qualified FBS | bFGF, hLIF | MEFs | EB | Teratoma | Lentivirus (Human OKSM) | G-banding / Normal | [91] | |
| Dermal fibroblasts (adult) | Knockout DMEM, 20% KSR | mLIF | MEFs | None | Germ cell-like tumor | Lentivirus (Human OKSMLN) | G-banding / Normal | [92] | |
| Skin fibroblasts (3 yrs) | DMEM/F12, 20% KSR | bFGF, hLIF, PD0325901, CHIR99021 | MEFs | EB | Teratoma | Retrovirus (Mouse OKSM) | Array-CGH and FISH /chromosome gains, CFA4, 8, 13 and 16 | [93] | |
| Pig | Primary ear fibroblasts Primary bone marrow (10 wks) | DMEM/F12, 20% KSR | Need continuous DOX | MEFs | EB | Teratoma | DOX-inducible Lentivirus (Human OKSM | G-banding / Normal | [77] |
| Embryonic fibroblasts (D37) | DMEM, 10% / 15% FBS | bFGF with 10% FBS or mLIF with 15%FBS | MEFs | None | Teratoma | Retrovirus (Human/Mouse OKSM) | G-banding / Normal | [71] | |
| Fetal fibroblasts | Knockout DMEM, 20% KSR | bFGF, 4% O2 | MEFs | EB | Teratoma | Lentivirus (Human OKSM) | G-banding / Paracentric inversion, Ch16 | [43] | |
| Mesenchymal stem cells | mTeSR1 (StemCell) | Matrigel | EB | Germline chimera | Lentivirus (Human OKSMLN) | None / None | [80] | ||
| Embryonic fibroblasts | DMEM/F12, 20% KSR | bFGF or LIF, PD0325901, CHIR99021, VPA | MEFs | EB | Teratoma | Episomal plasmids (Human OKSMNL) | None / None | [72] | |
| Ear fibroblasts (6 mos) | 1:1 mix of hES (20% KSR) and mES 20% (FBS) media | bFGF, LIF | MEFs / 0.1% gelatine | EB | Teratoma | Retrovirus (Mouse OKSM) | G-banding / Normal | [78] | |
| Ear fibroblasts (6 mos) | 1:1 mix of hES (20% KSR) and mES (20% FBS) media | bFGF, LIF | MEFs / 0.1% gelatine | EB | Teratoma | Retrovirus (Mouse KSM) | G-banding / Normal | [79] | |
| Embryonic fibroblasts | Knockout DMEM, 20% KSR | pLIF (conditioned medium), forskolin | Collagen I | EB | Chimeras | Retrovirus (Human OKSM) | G-banding / Normal | [73] | |
| Fetal fibroblasts | DMEM, 10% FCS | mLIF, N2B27, PD0325901, CHIR, PD173074, Need continuous DOX | MEFs | EB | None | DOX-inducible Lentivirus (Human OKSM) | Normal | [74] | |
| Fetal fibroblasts | Knockout DMEM, 15% KSR | bFGF, Doxycycline | MEFs | EB | None | DOX-inducible Lentivirus (Human OKSMN) | None | [75] | |
| Embryonic fibroblasts | DMEM/F10, 15% FBS | bFGF, hSCF | MEFs | EB | None | Transfection (Mouse OKSM) | G-banding / Normal | [76] | |
| Cow | Fetal fibroblasts | DMEM, 20% KSR | bFGF | MEFs | EB | Teratoma | Retrovirus (Bovine OKSM, Bovine OKSMLN, Human OKSM) | G-banding / Normal | [45] |
| Skin fibroblasts | MEMα, 20% FBS, ITS | Bovine FGF, hLIF | MEFs | EB | Teratoma | Retrovirus (Human OKSMN) | G-banding / Normal | [96] | |
| Fetal fibroblasts | 1:1 Mix of DMEMF/F12 + N2 and Neurobasal + B27 | hLIF, PD0325901, CHIR99021 | MEFs | EB | Teratoma | Episomal virus-free poly-promoter vector (Bovine OKSM) | G-banding / Normal | [95] | |
| Skin fibroblasts (2.5–4 mos) | DMEM, 15% FBS | LIF, bFGF | MEFs | EB | Teratoma | Lentivirus (Human O+ Pig KSM) | G-banding / Normal | [97] |