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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2013 Sep 27;12(12):10.1158/1535-7163.MCT-13-0439. doi: 10.1158/1535-7163.MCT-13-0439

Figure 3.

Figure 3

Metformin induces mislocalization of K-Ras. A) K-Ras localization following metformin treatment analyzed by confocal microscopy in Madin-Darby Canine Kidney (MDCK) cells expressing GFP-labeled oncogenic K-RasG12V mutant. B) Quantitative image analysis was performed to determine ratio of K-Ras at the plasma membrane versus total K-Ras (normalized to PBS-treated controls). Representative experiments shown, three independent experiments were performed. C) Immunoblot of Hec1A subcellular fractionation following 48 hours of metformin or AICAR treatment. D) Quantitation of fractionation immunoblot shows a concentration-dependent translocation of K-Ras from the membrane to the cytoplasm compared to PBS treatment. Error bars indicate +/− SEM. *p<0.05