TABLE I.
Results of Immunohistochemical Analyses in the Eight MDA PCa 144 Xenografts and the Pelvic Exenteration Specimen of Origin*
| # Blocks | # Cores |
% AR + Nuclear |
% PSA + Cytoplasmic |
Chr A + Cytoplasmic |
Syn + Cytoplasmic |
% Ki-67 + Nuclear |
p53 + Nuclear |
% PTEN + Cytoplasmic |
% pAKT + Cytoplasmic |
% bcl-2 + Cytoplasmic |
% Sox9 Nuclear |
% PTPRZ Cytoplasmic |
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PATIENT DONOR TUMOR TISSUE COMPONENTS | |||||||||||||
| ADENO | n/a | n/a | 90 | 40 | − | − | 30 | + | 10 | 20 | 20 | 80 | 10 |
| LCNEC | n/a | n/a | <1 | 5 | + | + | 95 | + | 0 | 60 | 70 | 60 | 60 |
| SCC | n/a | n/a | <1 | <1 | + | + | 95 | + | 0 | 10 | 40 | 15 | 70 |
| XENOGRAFTS | |||||||||||||
| 144-13 | 3 | 9 |
0.3±0.5 0(0-1) |
0±0 | + | + |
85±12 90(60-95) |
+ | 10±0 |
91±11 95(70-100) |
0±0 |
12±10 10 (0-40) |
99±2 100(95-100) |
| 144-11 | 2 | 6 |
0.8±2 0(0-5) |
0±0 | + | + | 40±0 | + |
1±0.5 1(0-1) |
75±19 75(50-100) |
8±13 0 (0-30) |
13±8 10(10-30) |
72±20 70(40-90) |
| 144-20 | 3 | 9 | 0±0 | 0±0 | + | + |
45±31 50(10-80) |
+ | 1±0 |
89±8 90(80-100) |
2±8 0 (0-30) |
0±0 |
75±20 70 (60-100) |
| 144-23 | 2 | 6 | 0±0 | 0±0 | + | + |
31±27 25(5-70) |
+ | 1±0 |
72±10 75(60-80) |
0±0 | 0±0 |
95±5 95 (90-100) |
| 144-4 | 3 | 9 | 0±0 | 0±0 | + | + |
95±0 95(95-95) |
+ |
11±14 5(0-30) |
93±14 100(60-100) |
41±21 40 (0-70) |
98±4 100(90-100) |
77±13 80(60-90) |
| 144-2 | 3 | 9 | 0±0 | 0±0 | + | + |
14±2 15(10-15) |
+ | 0±0 |
85±17 90(60-100) |
100±0 | 100±0 |
93±10 95(80-100) |
| 144-6 | 3 | 9 |
0.7±2 0(0-5) |
0±0 | + | + |
87.5±8.5 90(70-95) |
+ |
8±8 5(1-20) |
100±0 |
72±17 70(40-100) |
n/a |
88±14 90(60-100) |
| 144-9 | 1 | 3 |
5±5 0(0-10) |
0±0 | + | + |
43±15 40(30-60) |
+ |
4±5 1(1-10) |
100±0 |
2±2 1(1-5) |
83±8 85(70-90) |
75±7 75(70-80) |
Abbreviations: AR, androgen receptor; PSA, prostate-specific antigen; Chr A, chromogranin A; Syn, synaptophysin; Adeno, adenocarcinoma; LCNEC, large-cell neuroendocrine carcinoma; SCC, small-cell carcinoma.
*
Data are expressed for tissue microarray results as means ± standard deviations (SD) of positive staining. When SD>0, medians with ranges in parentheses are given below. For 4-μm tissue sections, results are given as positive (+) or negative (−).