TABLE 1. NQO1 activity in CHO cell lines transfected with human NQO1 cDNA.
Values are mean ± standard deviations of at least three independent determinations. NQO1 activity was measured in cell extracts by monitoring the dicumarol-inhibitable reduction of DCPIP at 600 nm using NADH as the electron donor substrate. NQO1 activity was stable in the transfected cell lines, and expression was maintained in the absence of G418 selection.
a
NQO1 activity is expressed in nmol of DCPIP reduced/min/mg of protein.
b
n.d. = not detectable.