TABLE 2. Toxicity of MMC, MeDZQ, STPN, and EO9 to CHO, DTD812, and DTD818 as measured with the MTT assay.
IC50 ((dose resulting in a 50% reduction in absorbance at 550 nm) values are mean ± standard deviations of three independent determinations. Cells (~5 × 104) were treated for 2 hr with graded concentrations of MMC, MeDZQ, STPN, or EO9, and MTT formazan crystal staining was determined 5–7 days after treatment. IC50 values were determined from semilog plots of percentage of control absorbance at 550 nm versus concentration of drug.
| Cell line | IC50 |
|||
|---|---|---|---|---|
| MMC | MeDZQ | STPN | EO9 | |
| nm | ||||
| CHO | 1360 ± 140 | 289 ± 2 | 98 ± 19 | 625 ± 9 |
| DTD812 | 1560 ± 230 | 71 ± 26 | 58 ± 9 | 215 ± 81 |
| DTD818 | 1900 ± 340 | 73 ± 3 | 65 ± 9 | 223 ± 50 |