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. 2014 Jan 7;12(1):e1001746. doi: 10.1371/journal.pbio.1001746

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Overexpression of PknB by the tet-pknB mutant upon induction with ATc. PknB levels were determined by Western blot using PknB-specific rabbit IgG and anti-FLAG antibody. (B) Effects of PknB overexpression measured by CFU for 6 d post-ATc induction and by (C) optical density in comparison to the kinase dead mutant tet-pknB K40A. (Inset) PknB expression in the tet-pknB and tet-pknB K40A mutants was determined by anti-FLAG Western Blot. 1, wild type; 2, tet-pknB; 3, tet-pknB K40A, all treated with 20 ng/ml ATc. Error bars represent standard deviation.