Table 2. Summary of variant calling following DNA sequencing.
WGA tumor | TotalHetero | TotalHomo | RatioHetero/homo | Transition/Transversion rate | Coverage intarget regions | Totalreads |
118(T) | 7359 | 4136 | 1.78 | 3.42 | 39.67 | 35.55 M |
127(T) | 8068 | 4627 | 1.74 | 3.32 | 72.28 | 37.43 M |
140(T) | 7904 | 4411 | 1.79 | 3.25 | 59.33 | 34.73 M |
146(T) | 8104 | 4247 | 1.91 | 3.23 | 61.05 | 34.47 M |
210(T) | 7806 | 4333 | 1.80 | 2.96 | 53.72 | 54.23 M |
225(T) | 8088 | 4258 | 1.90 | 2.91 | 83.70 | 43.54 M |
247(T) | 7091 | 3498 | 2.03 | 2.58 | 24.65 | 19.77 M |
255(T) | 7387 | 4215 | 1.75 | 3.29 | 62.69 | 33.76 M |
278(T) | 8328 | 4612 | 1.81 | 3.11 | 107.10 | 57.96 M |
295(T) | 7517 | 4451 | 1.69 | 3.24 | 48.06 | 28.13 M |
322(T) | 9019 | 4173 | 2.16 | 2.53 | 49.57 | 19.14 M |
344(T) | 8107 | 4300 | 1.89 | 3.08 | 59.31 | 29.42 M |
396(T) | 7408 | 4169 | 1.78 | 3.06 | 31.25 | 35.00 M |
412(T) | 8755 | 4752 | 1.84 | 2.84 | 98.08 | 45.19 M |
421(T) | 8552 | 4489 | 1.91 | 2.95 | 81.62 | 37.04 M |
541(T) | 9669 | 4511 | 2.14 | 3.11 | 35.20 | 37.75 M |
Average | 8073 | 4324 | 1.87 | 3.06 | 60.46 | 36.44 M |
StDev | 683 | 286 | 0.14 | 0.25 | 23.45 | 10.40 |
Unamplified tumor | ||||||
118(T) | 9006 | 4823 | 1.87 | 2.95 | 48.39 | 26.08 M |
127(T) | 14029 | 3152 | 4.45 | 3.27 | 87.78 | 32.40 M |
140(T) | 8725 | 4875 | 1.79 | 3.16 | 64.98 | 35.76 M |
146(T) | 15193 | 3123 | 4.86 | 3.18 | 85.07 | 29.08 M |
210(T) | 8860 | 5117 | 1.73 | 3.17 | 79.28 | 26.47 M |
225(T) | 14270 | 3081 | 4.63 | 3.30 | 73.46 | 24.50 M |
247(T) | 8879 | 5066 | 1.75 | 3.22 | 108.08 | 53.61 M |
255(T) | 8743 | 4891 | 1.79 | 3.26 | 81.93 | 42.86 M |
295(T) | 8717 | 5067 | 1.72 | 3.03 | 54.27 | 110.79 M |
278(T) | 8656 | 4998 | 1.73 | 3.21 | 88.89 | 47.00 M |
322(T) | 8650 | 4991 | 1.73 | 3.11 | 73.08 | 37.62 M |
344(T) | 14161 | 3006 | 4.71 | 3.15 | 77.05 | 26.14 M |
396(T) | 7627 | 4455 | 1.71 | 3.27 | 36.48 | 17.41 M |
412(T) | 8473 | 4940 | 1.72 | 3.21 | 73.48 | 38.37 M |
421(T) | 9085 | 4977 | 1.83 | 3.14 | 81.82 | 45.31 M |
541(T) | 10900 | 5329 | 2.05 | 3.17 | 73.85 | 37.67 M |
Average | 10248 | 4493 | 2.50 | 3.18 | 74.24 | 39.44 M |
StDev | 2574 | 855 | 1.29 | 0.09 | 17.11 | 21.30 |
WGA normal | ||||||
118(N) | 7476 | 4154 | 1.80 | 3.43 | 73.07 | 38.26 M |
127(N) | 9290 | 4619 | 2.01 | 2.64 | 101.20 | 23.63 M |
140(N) | 7288 | 3984 | 1.83 | 3.41 | 59.76 | 35.17 M |
146(N) | 8292 | 4613 | 1.80 | 3.32 | 67.14 | 37.40 M |
210(N) | 7894 | 4334 | 1.82 | 3.13 | 48.37 | 39.87 M |
225(N) | 11154 | 4935 | 2.26 | 2.07 | 127.39 | 75.50 M |
247(N) | 8925 | 4940 | 1.81 | 3.27 | 123.26 | 37.12 M |
255(N) | 8560 | 4771 | 1.79 | 3.25 | 83.78 | 26.35 M |
295(N) | 6506 | 3414 | 1.91 | 3.18 | 27.59 | 29.18 M |
278(N) | 6961 | 3966 | 1.76 | 3.10 | 30.67 | 28.21 M |
322(N) | 7747 | 4610 | 1.68 | 3.29 | 56.95 | 25.81 M |
344(N) | 8082 | 4298 | 1.88 | 3.09 | 60.13 | 17.95 M |
396(N) | 7276 | 4035 | 1.80 | 3.18 | 27.70 | 34.08 M |
412(N) | 8010 | 4156 | 1.93 | 2.84 | 38.52 | 26.65 M |
421(N) | 8561 | 4579 | 1.87 | 3.11 | 64.07 | 30.20 M |
541(N) | 10411 | 4840 | 2.15 | 3.03 | 78.53 | 46.44 M |
Average | 8277 | 4391 | 1.88 | 3.08 | 66.76 | 34.49 M |
StDev | 1224 | 423 | 0.15 | 0.34 | 30.79 | 13.08 |