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. 2014 Jan 7;9(1):e84996. doi: 10.1371/journal.pone.0084996

Figure 2. Characterization of isoform specific SLC2A9 antibodies.

Figure 2

30 µg of COS cell lysate transfected with mock or SLC2A9s were separated by SDS-PAGE and Western blotting was performed with anti-SLC2A9-S (A), antigen pre-absorbed anti- SLC2A9-S (B), anti-SLC2A9-L (C), or antigen pre-absorbed anti-SLC2A9-L (D) antibodies. COS cells were transfected with GFP tagged SLC2A9-S (E and K) or SLC2A9-L (H and N) and immunofluorescence was performed with anti-SLC2A9-S (F and I) or anti-SLC2A9-L (L and O) antibodies. Overlay images were shown in G, J, M and P. The scale bar of 40 µm was shown in K.