Figure 6. Significance of the putative di-leucine motif in SLC2A9-L on basolateral sorting.

A. GFP-tagged Long_33-34AA (green), in which 33^rd and 34^th leucine-leucine was changed to alanines were expressed in MDCK cells. B. GFP-tagged Long_32-36del (green), in which 32^nd–36^th amino acids including the putative di-leucine motif was deleted, were expressed in MDCK cells. Cell surface of the apical or basolateral membrane was biotinylated and visualized in red. Confocal images of xy and z axes were obtained and the magnification is the same as Fig. 3.