Figure 7. Effect of N-terminal deletion or exchange of SLC2A9-L.

Long_25AAdel (A) or Long_30AAdel (B) constructs, in which N-terminal 25 or 30 amino acids were deleted from SLC2A9-L, respectively, Long_27-34A (C), in which amino acids from 27^th and 31st were changed to alanines, or Long_27-31del (D), in which 5 amino acids from 27^th and 31^st were deleted, were transfected into MDCK cells. These GFP-tagged constructs were visualized in green. Cell surface of the apical or basolateral membrane was biotinylated and visualized in red. Confocal images of xy and z axes were obtained and the magnification is the same as Fig. 3.