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. 2014 Jan 7;9(1):e84982. doi: 10.1371/journal.pone.0084982

Figure 3. Effects of DUSP1 knockdown on gemcitabine response in human pancreatic cancer cells.

Figure 3

AsPC-1, BxPC-3, and COLO-357 cells were stably transduced with lentivirus expressing shRNA against scramble control or DUSP1. (A) Cells were incubated for 48 h in the absence or presence of varying concentrations of gemcitabine, and MTT assays were performed. Data are the means ± SEM of 3 experiments. *p<0.05; **p<0.01, compared with control. (B) AsPC-1, BxPC-3, and COLO-357 cells were incubated for the indicated times with 100 ng/ml, 10 ng/ml, and 5 ng/ml gemcitabine, respectively, and analyzed by immunoblotting.