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. 2014 Jan 7;9(1):e84982. doi: 10.1371/journal.pone.0084982

Figure 7. Effects of combining gemcitabine with DUSP1 knockdown on animal survival and tumor growth.

Figure 7

Immunodeficient mice carrying orthotopic tumors derived from COLO-357 cells which stably express doxycycline-inducible shRNA against scramble control or DUSP1 were given doxycycline in the drinking water and treated with vehicle control (saline) or gemcitabine (50 mg/kg, i.p., twice weekly). (A) Kaplan-Meier survival. *p<0.05, compared with scramble treated with gemcitabine; ##p<0.01, compared with scramble treated with saline. (B) Q-PCR measurement of DUSP1 mRNA levels. (C) TUNEL staining and immunohistochemical analysis of CD34, Ki67, and cleaved caspase 3. Scale, 20 µm. (D) Quantification. Data are the means ± SEM of 3 experiments. *p<0.05; **p<0.01, compared with scramble treated with saline.