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. 2014 Jan 7;9(1):e85348. doi: 10.1371/journal.pone.0085348

Figure 1. Time schedules and experimental designs.

Figure 1

Male Wistar rats were sham injected at least two times prior to LPA treatment to familiarize animals with the i.c.v. procedure. The open field test (OFT) and elevated plus maze (EPM) were performed under novelty (A) and habituation (B) conditions after 5 min of LPA 18∶1 infusion. For habituation, the rats were sham injected and tested in both paradigms 24 h before LPA infusion. Rats were exposed to the sample trial of the Y maze (YMT) 5 min after LPA 18∶1 administration, and performed the test trial 2 h later (C). The forced swimming test (FST) was performed after 5 min of LPA 18∶1 infusion (D). Food and water intake were evaluated at different times in 24 h food-deprived animals after 5 min of LPA 18∶1 infusion (E). c-Fos immunoreactivity (IR) was performed through perfusion after 90 min of LPA 18∶1 infusion (F).