Figure 2. Wistar rats were studied in the elevated plus maze (EPM) and open field test (OFT) under novelty and habituation conditions following LPA 18∶1 infusion at doses of 0, 0.4 and 2 µg.

In the EPM, the time (s) exploring the exposed arms (A) and the total number of arm entries (B) were evaluated under novelty conditions. Similarly to the novelty conditions, the time exploring the open arms (C) and the total arm entries (D) were evaluated in animals previously habituated to the EPM (A). Locomotor activity was measured based on the number of crossings (E) and the time (%) spent in the center of the field (F) under novelty conditions. Again, both the number of crossings (G) and the percentage of time spent in the center (H) were evaluated under habituation conditions. The bars are the means ± SEM (n = 7–12 animals per group). The data were analyzed using one-way ANOVA. *p<0.05 and ***p<0.001 denote significant differences versus the vehicle-treated group, determined using Bonferroni’s post-hoc test.