Figure 3. Multiple TRIP8b splice variants are expressed in photoreceptors.

A) Mouse retina section before laser capture micro-dissection, dotted white lines indicate region subsequently collected and shown in B. C) RT-PCR products obtained using RNA isolated from tissue collected as in B with the isoform specific primers depicted in Figure 2A. Reverse transcriptase was omitted as a negative control. Immunostaining with an antibody recognizing exon 4 in TRIP8b labels photoreceptors of D) wild type, E) TRIP8b 1b/2^−/−, but not F) TRIP8b^−/− mouse retina. Asterisks indicate non-specific labeling of blood vessels. Abbreviations as in Figure 1; Scale bars are 20 µm.