Figure 5. HCN1 protein levels are reduced in the absence of TRIP8b.

Anti-HCN1 antibodies were used to immunostain retina from A) wild type B) TRIP8b 1b/2^−/−, or C) TRIP8b^−/− mice. Abbreviations as in Figure 1; Scale bars are 20 µm. D) Western blots of total retina lysates probed with anti-HCN1, with PDC used as a loading control. E) Relative amount of HCN1 expressed in the retina of wild type, HCN1 heterozygous, and TRIP8b^−/− mice as measured using semi-quantitative Western blots.