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. 2014 Jan 7;9(1):e85850. doi: 10.1371/journal.pone.0085850

Figure 7. The surface expression of HCN1 is maintained in TRIP8b−/−.

Figure 7

A) Streptavidin staining of control (left) and biotinylated retina (right). B) After biotinylation, surface proteins from either control wild type (WT) or TRIP8b−/− mice were pulled down using NeutrAvidin beads. The level of HCN1 in the total (input) and surface (eluted from NeutrAvidin beads) pools was detected by Western blotting. PDC and NKA were used as negative and positive controls, respectively. C) Densitometry of Western blots represented in (B) was used to calculate the surface to total ratio of HCN1 after normalization to the loading control NKA.