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Fluorescence ratio F560/F488 measured in FIRE-1 expressing ESdCs superfused with A: the PLC inhibitor, U73122 (1 µmol/L; n = 5), B: its inactive analog, U73343 (1 µmol/L; n = 2), C: the InsP3R blocker, 2-APB (2 µmol/L; n = 2), D: Ca2+ -free solution (n = 2) or E: BAPTA-AM. F: The normalized changes of F560/F488 recorded in ET-1, PE or U73122. #: one way ANOVA P<0.05.
