Table 5. Percentage distribution of methylated and unmethylated CpG zebrafish RRBS samples and comparison with zebrafish WGBS*.
| Sample | Unmethylated (0–20% methylation) CpG | Intermediate methylation (20.1–79.9%) | Methylated (80–100% methylation) |
|---|---|---|---|
| Male1 (RRBS) |
15.5 |
15.6 |
68.9 |
| Male2 (RRBS) |
16.4 |
16.9 |
66.7 |
| Female1 (RRBS) |
19.1 |
14.5 |
66.4 |
| Female2 (RRBS) |
17.0 |
18.6 |
64.4 |
| Liver (RRBS) |
23.8 |
12.8 |
63.4 |
| Sperm (WGBS) |
5.0 |
1.2 |
93.8 |
| Egg (WGBS) |
6.3 |
29.7 |
64.0 |
| Muscle (WGBS) |
4.1 |
26.7 |
69.2 |
| Sphere† (WGBS) | 5.7 | 0.80 | 93.5 |
*
The methylation percentages for zebrafish brain (Male1–2 and Female 1–2) and liver RRBS samples were calculated on CpG10 as part of the current study. Percentages for zebrafish sperm, egg, muscle and sphere were derived from recently published whole genome bisulfite sequencing.20 †Four hour post-fertilization.