Figure 4.MAGEA11 expression and methylation and global DNA methylation. (A) MAGEA11 expression and LINE1 methylation in human prostate cell lines. MAGEA11 expression was determined by RT-qPCR and LINE1 methylation was determined by pyrosequencing. PWR-1E and RWPE-2 are derived from normal prostate epithelium, while other cell lines are derived from prostate cancer. Spearman test showed an inverse association between MAGEA11 expression and LINE1 methylation, which did not reach statistical significance (R = -0.455, p = 0.267). (B) MAGEA11 expression and LINE1 methylation in primary prostate tissues. Measurements were determined as described in (A). (C) MAGEA11 expression and LINE1 methylation in normal ovary and EOC with divergent LINE1 methylation. MAGEA11 expression and LINE1 methylation were determined as described in (A). MAGEA11 expression is plotted on a log axis and note that five samples in the hypermethylated EOC group did not express measurable MAGEA11. The mean LINE1 methylation level in three sample groups is shown below the x-axis. Two-tailed t-test results are shown. (D) MAGEA11 expression and LINE1 methylation are indirectly associated in EOC. MAGEA11 expression was plotted vs. LINE1 methylation in EOC samples from both EOC groups shown in (C). Spearman test results are shown. (E) MAGEA11 TSS methylation and LINE1 methylation are directly associated in prostate cancer. Methylation was determined using bisulfite sequencing (MAGEA11) and pyrosequencing (LINE-1). Spearman test results are shown. (F) MAGEA11 TSS methylation and LINE1 methylation are directly associated in EOC. Methylation was determined using pyrosequencing. Spearman test results are shown.