Figure 5. Epigenetic modulatory drugs induce MAGEA11 expression. MAGEA11 expression was measured by RT-qPCR following treatment of benign prostate or prostate cancer cell lines with vehicle (PBS/DMSO), Trichostatin A (TSA), and/or decitabine (DAC), as described in Materials and Methods. (A) DU145 cells. (B) PC-3 cells. (C) LAPC-4 cells. (D) PWR-1E cells. We noted that the MAGEA11/GAPDH copy number in control LAPC-4 cells appeared substantially lower than observed in other experiments, likely due to repression by DMSO. This effect was also observed in Figure 7C.