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. Author manuscript; available in PMC: 2015 Feb 26.
Published in final edited form as: Vaccine. 2013 Jul 1;32(10):1218–1224. doi: 10.1016/j.vaccine.2013.06.056

Fig. 3.

Fig. 3

Electrophoretic analysis of LecA preparations. The left lane in each pattern contained the same molecular markers with the designated molecular mass shown on the left. Lanes 1–3 represented Lot #s 071111RSW, 071111LAB, and 071411LAB, respectively. For SDS-PAGE analysis, each lane contained 10 μg protein. For silver stain and immunoblotting with 7F4 MAb and 3F4 MAb, Lanes 1–3 each contained 2.5 μg protein. SDS-PAGE for silver staining was performed in 0.01 M MES buffer, pH 7.5, accounting for the slightly slower migration of the proteins. Native gel analysis was performed using 10 μg protein per lane.