Table 1.
Protein purification and analysis of purified LecA preparations. Assays were performed in duplicate and represent average values. The total protein yield for Lot #071111LAB was lower than the other two lots because only half of the starting material was processed. The extrapolated yield for this particular lot was comparable to that observed with the other two lots.
| Lot # | Total proteina (mg) | Endotoxinb (EU/μg) | DNAc (ng/mg) | RNAc (ng/mg) | Analytical sensitivityd (ng detected) |
Linear regression analysisd |
|---|---|---|---|---|---|---|
| 071111RSW | 27.0 | 0.01 | 51 | <20 | 0.14 | 0.71 |
| 071111LAB | 12.9 | 0.01 | 82 | <20 | 0.11 | 0.77 |
| 071411LAB | 24.7 | 0.02 | 61 | <20 | 0.31 | 0.74 |
Protein was determined using the Thermo Scientific/Pierce Modified Lowry Assay (Rockford, IL).
Endotoxin levels were determined using the FDA-licensed Endosafe PTS™ cartridges and reader from Charles River Laboratories.
Double-stranded (ds) DNA and RNA were quantified using the Qubit™ Fluorimeter and Life Technologies kits.
Analytical sensitivity and linear regression analysis was determined using a modified quantitative E. HISTOLYTICA II kit.