Skip to main content
. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 19;0:370–383. doi: 10.1016/j.freeradbiomed.2013.04.021

Figure 4. Alterations in the redox state of cytosol and mitochondrial compartments in response to parkinsonian neurotoxins.

Figure 4

In A, stable cells overexpressing roGFP and Mito-roGFP were stained with MitoTracker Red to depict mitochondrial localization of Mito-roGFP. In B, roGFP and Mito-roGFP cells were treated with paraquat, MPP+ or rotenone as indicated. Cells were co-stained with PI and only viable cells were analyzed (see population of PI- cells in the grey region of the histogram). Alterations in the redox state were determined by ratiometric analysis of changes in (Mito-)roGFP fluorescence at 407/488 ex and 530 em normalized with respect to control values. Data in graphs represent means ± SE of at least five independent experiments. *p<0.05 vs control values.