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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Methods. 2013 Jun 20;66(3):433–440. doi: 10.1016/j.ymeth.2013.06.012

Fig. 5. QF and Gal4 activation from a dual reporter construct.

Fig. 5

(A) Co-injection of the pBT2QUAS:dtomato-4xnrUAS:GFP dual reporter with QF (pT2Kubb QF) and Gal4 (pT2KEF1 Gal4) driver constructs results in mosaic labeling, with some cells expressing dTomato, GFP, or both fluorescent proteins (white arrowheads, 2 dpf, lateral view). (B) dTomato labeled motoneuron (white arrowhead) and (C) GFP labeled hindbrain neuron (white arrowhead) in the same 3 dpf larva, following injection of the dual reporter plasmid into a Tg(mnx1:QF)/+; Tg(ptf1:Gal4)/+ doubly transgenic embryo.