Skip to main content
. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 30;0:428–437. doi: 10.1016/j.freeradbiomed.2013.04.031

Fig. 10. Effect of Cp siRNA and human oxy-Hb on nitrite and SNO formation in A549 cells.

Fig. 10

A549 cells were transfected with Cp siRNA (1 µM) or scrambled siRNA control by using Accell delivery media (Thermo Scientific) in 12-well plates with cells of 1×105/ml/well. After transfection, the complete culture medium was replaced with serum deprived DMEM medium and human oxy-Hb (20 µM) was added to the cells together with LPS/cytokine mixture for 24 hrs. Nitrite levels in conditioned media (A) and cells (B), as well as SNO production in cells (C) were measured with NOA. *ρ < 0.05 and **ρ < 0.01 compared to controls.