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. Author manuscript; available in PMC: 2014 Oct 13.
Published in final edited form as: Free Radic Biol Med. 2013 Apr 30;0:428–437. doi: 10.1016/j.freeradbiomed.2013.04.031

Fig. 8. NO was scavenged by human oxy-Hb in a dose-dependent manner with little effect on iNOS expression.

Fig. 8

A549 cells (5×105/ml/well) were plated in 12-well plates and cultured with complete DMEM growth medium for 24 hrs. The medium was then replaced with serum deprived DMEM medium before treated with different concentrations of human oxy-Hb for 24 hrs in the presence of LPS/cytokine mixture. Nitrite levels in conditioned media were measured with Sievers 280i NOA (A, B). iNOS expression was determined by Western blotting (C).