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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Oncogene. 2013 Mar 11;33(9):1181–1189. doi: 10.1038/onc.2013.42

Figure 4.

Figure 4

ARHGAP5 is the target gene of miR-486-5p. (a) a miR-486-5p target site within 3′-UTR of ARHGAP5 was predicted by bioinformatic algorithms. (b) A luciferase reporter assay in A549 cells showed that luciferase activity controlled by ARHGAP5-3′-UTR was dramatically inhibited by ectopic miR-486-5p expression. (c) Top, H157 cells had a high expression level of miR-486-5p after transfection with miR-486-5p mimic. Bottom, AHGAP5 expression level was reduced in the H157 cells transfected with miR-486-5p mimics determined by western blotting with p190-B antibody. (d) Top, A549 cells had a high expression level of miR-486-5p after transfection with miR-486-5p mimic. Bottom, the level of ARHGAP5 protein expression was reduced in A549 cells transfected with the miR-486-5p mimic.