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. Author manuscript; available in PMC: 2014 Aug 27.
Published in final edited form as: Oncogene. 2013 Mar 11;33(9):1181–1189. doi: 10.1038/onc.2013.42

Figure 5.

Figure 5

Reduced expression of ARHGAP5 inhibits cell proliferation, migration and invasion. (a) Top, the transcription level of ARHGAP5 was significantly inhibited by si-ARHGAP5 in A549 cells as determined by qRT–PCR. Bottom, ARHGAP5 protein was repressed by the small interfering RNA at 48 h in A549 cells as determined by western blotting with p190-B antibody. (b) Top, methylthiazol tetrazolium assay showed that cell proliferation was decreased in A549 cancer cells transfected with si-ARHGAP5 compared with cells with scrambled sequence. Bottom, Colony formation assay demonstrated that cell growth of A549 cells was repressed due to downregulation of ARHGAP5. (c) Wound-healing assay indicated that ARHGAP5 downregulation inhibited cell migration and invasion of A549 cells. (d) Transwell assay showed that knockdown of ARHGAP5 restrained cell migration and invasion of H157 cells.