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. Author manuscript; available in PMC: 2014 Sep 5.
Published in final edited form as: Free Radic Biol Med. 2013 Mar 5;0:179–196. doi: 10.1016/j.freeradbiomed.2013.02.006

Fig. 11.

Fig. 11

Effects of exposure to CrVI on mRNA expression of GSTM1, GSTM2, GSTA4, and GSHR in GCs, SIGCs, and TCs. GCs and TCs were isolated from control immature rats (PND 23–26), as described under Materials and methods. GCs, SIGCs, and TCs were cultured and treated with CrVI with or without vitamin C pretreatment, as described in the legend to Fig. 7. Histograms depict expression of GSTM1 mRNA in (A) GCs, (B) SIGCs, and (C) TCs; GSTM2 mRNA in (D) GCs, (E) SIGCs, and (F) TCs; GSTA4 mRNA in (G) GCs, (H) SIGCs, and (I) TCs; and GSHR mRNA in (J) GCs, (K) SIGCs, and (L) TCs. CrVI treatment decreased mRNA expression of GSTM1, GSTM2, GSTA4, and GSHR, and vitamin C pretreatment mitigated or inhibited the effects of CrVI in GCs and SIGCs. In TCs, vitamin C failed to mitigate the effects of CrVI on GSTM1 and GSTM2. Each value is the mean±SEM of three different samples per treatment, P<0.05. aCrVI treatment, 0 h vs 12 or 24 h; bCrVI+vitamin C (pretreatment), 0 h vs 12 or 24 h; cCrVI (12 or 24 h) vs CrVI+vitamin C (12 or 24 h).