Effects of exposure to CrVI on mRNA expression of TXN1, TXN2, TXNRD2, and PRDX3 in GCs, SIGCs, and TCs. GCs, SIGCs, and TCs were cultured and treated with CrVI with or without vitamin C pretreatment, as described in the legend to Fig. 7. Histograms depict expression of TXN1 mRNA in (A) GCs, (B) SIGCs, and (C) TCs; TXN2 mRNA in (D) GCs, (E) SIGCs, and (F) TCs; TXNRD2 mRNA in (G) GCs, (H) SIGCs, and (I) TCs; and PRDX3 mRNA in (J) GCs, (K) SIGCs, and (L) TCs. CrVI treatment decreased mRNA expression of TXN1, TXN2, TXNRD2, and PRDX3 and vitamin C mitigated or inhibited the effects of exposure to CrVI in GCs and SIGCs. In TCs, vitamin C failed to mitigate effects CrVI treatment on TXN1 and TXN2. Each value is the mean±SEM of three different samples per treatment, P<0.05. aCrVI treatment, 0 h vs 12 or 24 h; bCrVI+vitamin C (pretreatment), 0 h vs 12 or 24 h; cCrVI (12 or 24 h) vs CrVI + vitamin C (12 or 24 h).