Fig. 1.

Expression of DGKη in lung cancer cell lines and in a mouse model of mutant EGFR lung cancer. a Lung cancer cell lines were grown to confluence, harvested, and then the levels of DGKη and actin were detected in the cell lysates by Western blotting. The mutation status of EGFR or K-Ras in each cell line is shown below the blot (mut mutation, wt wild-type). The type of lung carcinoma from which each line was obtained is also indicated (AD adenocarcinoma, LC large cell carcinoma). b Lungs from rtTA(−) × EGFR-L858R (control) or rtTA(+) × EGFR-L858R (L858R) mice treated with doxycycline for 8 weeks were harvested, fixed, sectioned, and stained with eosin. c Lung sections from control or L858R mice were immunostained to detect EGFR, PCNA, or DGKη