Figure 1.
CCI4 + ethanol–induced hepatocellular carcinoma (HCC) Chinese Kun Ming (KM) mouse were treated with CCI4 + ethanol and autopsied after natural death and killed. Histological examination of liver sections by H&E staining showed that the induction of hepatic cancer involves four stages: stage 1, hepatic steatosis occurred at 5 to 8 weeks; stage 2: hepatic cirrhosis occurred at 9 to 12 weeks; stage 3, hepatic precancerous lesion occurred at 13 to 16 weeks; stage 4, hepatic carcinoma occurred at 17 to 24 weeks. Photomacrographs are liver representative of at least two observations.