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. 2013 Dec 6;13:205. doi: 10.1186/1471-2229-13-205

Table 4.

MSAP fragment number analysis

Ploidy level Species E1/H1
E1/H3
Total
Hpa II Msp I Hpa II Msp I Hpa II Msp I
Diploid
L. lanceolatum
52
52
47
38
99
90
L. nydeggeri
50
48
43
33
93
81
L. ovalifolium
48
43
42
28
90
71
Tetraploid L. dodartii
37
77
24
56
61
133
L multiflorum
33
59
30
35
63
94
L. vulgare 26 46 20 39 46 85

Average number of MSAP fragments per diploid (L. lanceolatum, L. nydeggeri, L. ovalifolium) and tetraploid (L. dodartii, L. multiflorum and L. vulgare) species obtained using isoschizomers enzymes HpaII (methylation sensitive) and MspI (methylation insensitive) and primer combinations E1/H1 and E1/H3.