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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Methods. 2013 Jun 20;66(3):398–409. doi: 10.1016/j.ymeth.2013.06.009

Figure 3. Output from fastqc software for RNA-seq data quality analysis.

Figure 3

(A) Quality score per base position for reads. x-axis represents base pair position and y-axis represents interquartile range of quality value (from 0 to 40). Note the overall reduction in quality of scores toward the end of the 76 bp reads. (B) Graphical summary of quality scores shown in (A). (C) G/C distribution over all sequence reads represented on x-axis, with total reads corresponding on y-axis. Overall distribution (red line) versus the theoretical distribution in sample (blue line).