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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Methods. 2013 Jun 20;66(3):398–409. doi: 10.1016/j.ymeth.2013.06.009

Figure 4. Determining morpholino (MO) efficacy by RNA-seq.

Figure 4

(A) RT-PCR of cDNA generated from wild type and casz1 MO hearts shows aberrant splicing of casz1 intron 8 in MO-treated embryos. (B) Modified IGV viewer screenshot with schematic of casz1 mRNA (boxes) with inclusion of intron 8. Arrows indicate primers used for PCR shown in (A). Overall read coverage and individual reads mapping to casz1 mRNA in wild type and casz1 MO hearts are shown below schematic. Exon-intron boundaries predicted by the program Tophat based on read coverage are displayed on genome browser.