FIG 2.

mb-PMCA of 127S prions by using different concentrations of PrP^C substrate. Brain homogenate from tg338 mice infected with 127S prions was serially diluted in substrate containing either pure tg338 mouse brain homogenate (BH) or tg338 BH mixed with PrP^0/0 BH or PMCA buffer, as indicated. The mixture was then subjected to a single round of mb-PMCA. All samples were digested with PK before Western blot analysis. Undigested normal brain homogenate (PrP^C) and PK-digested, infected brain homogenate (PrP^res) are provided as electrophoretic references. Molecular mass markers (kDa) are indicated on the left.