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. 2013 Dec 31;5(1):e00829-13. doi: 10.1128/mBio.00829-13

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Copyright © 2013 Moudjou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — Western blot analysis of thermolysin-resistant PrP^Sc generated by mb-PMCA. Brain homogenate from tg338 mice infected with 127S prions was serially diluted in uninfected tg338 brain lysate, as indicated, and submitted to a single round of mb-PMCA. The resulting products were treated with either PK (+PK) or thermolysin (+TL) before Western blot analysis. The thermolysin-resistant signals were quantified so as to measure the ratio of thermolysin-resistant PrP^Sc to total PrP present in the unseeded samples (U). The ratios are indicated (% PrP^Sc). Unseeded sample has been 10-fold diluted before loading on the Western blot. The figure is representative of the ratios observed in two independent experiments. Molecular masses (kDa) are indicated at left.