FIG 5.
Endpoint titration of nonamplified and mb-PMCA-generated 127S prion infectivity in tg338 mice. Brain homogenates from infected tg338 mice and mb-PMCA-generated amplicons were 10-fold diluted (y axis) and inoculated intracerebrally into groups of tg338 mice. Individual survival times are shown on the x axis as days postinoculation. tg338 mice were inoculated with brain-derived 127S prions (filled triangles) or mb-PMCA amplicons generated from 10−5 (open circles), 10−7 (open squares), and 10−9 (open triangles) input seed.