FIG 6.
PrPres glycopattern and PrPres regional deposition in the brains of tg338 mice inoculated with mb-PMCA-generated 127S prions. Western blot analysis (a) and ratios (b) of high- and low-molecular-mass PrPres glycoforms in the brains and spleens of tg338 mice following challenge with brain-derived or mb-PMCA-generated 127S prions. Samples in panel a are from mice inoculated with 10-fold-diluted amplicons. Data in panel b are plotted as means ± standard errors of the means. Brains and spleen are represented by filled and open symbols, respectively. Brain-derived 127S prions are represented by red triangles; mb-PMCA amplicons generated from 10−5, 10−7, and 10−9 input seed are indicated by blue circles, squares, and triangles, respectively. The ratios were established from tissue samples of mice inoculated with all the amplicon dilutions. As no differences were observed among the amplicon dilutions, the ratios were combined for the sake of clarity. Histoblotting of representative coronal sections of tg338 mice inoculated with mb-PMCA amplicons at the level of the septum (c), hippocampus (d), midbrain (e), and brain stem (f) (10−9 input seed) and hippocampus (g and i) and midbrain (h and j) (10−7 and 10−5 input seed).