Fig. 3.

Recognition of the HLA-G α₁–α₃ structure by LILRB1 and LILRB2. a Immunoprecipitation of HLA-G1 and HLA-G5 (α₁–α₂–α₃ domains), and HLA-G2 and HLA-G6 (α₁–α₃ domains) with LILRB2-Fc. b Differential direct binding of HLA-G6-GST recombinant protein to LILRB1-Fc and LILRB2-Fc-coated beads. c Differential binding of B2M-free HLA-G5-GST and HLA-G6-GST recombinant proteins to NKL-LILRB1⁺ and NKL-LILRB1⁺LILRB2⁺ cells by flow cytometry analysis, using GST recombinant protein as control and anti-GST antibody for detection