Table 1.
Summary of Panx1 protein interaction partners, supporting evidence, functional significance, and relevant references.
| Interactor | Evidence | Function | Reference |
|---|---|---|---|
| Ion channels and receptors (and signaling complexes) | |||
| Pannexin 2 (Panx2) | MYC IPs (OE) | Modification of Panx1 channel | Bruzzone et al. (2003, 2005) |
| Functional coupling (OE) | function | Penuela et al. (2009) | |
| Panx1 and Panx2 IPs (OE) | Panx2 trafficking and localization | Ambrosi et al. (2010) | |
| Purified channels (OE) | |||
| Pannexin 3 (Panx3) | Co-loc (OE and Endg) | Unknown | Penuela et al. (2009) |
| Panx1 and Panx3 IPs (OE) | |||
| Purinergic receptor, P2X, ligand-gated ion channel 7 (P2X7 receptor) | MYC IP (OE) | Panx1 forms the large pore of the P2X7 receptor complex known as the “death pore” Inflammasome activation | Pelegrin and Surprenant (2006), Locovei et al. (2007), Iglesias et al. (2008), Silverman et al. (2009), Poornima et al. (2012), Xu et al. (2012), Kanjanamekanant et al. (2013), Wang et al. (2013) |
| Functional coupling (OE) | |||
| EGFP IP (OE) | |||
| Panx1 and P2X7 IPs | |||
| P2X7 IP | |||
| Panx1 IP | |||
| MYC and FLAG IPs (OE) | |||
| Inflammasome (associated with P2X7) | Inflammasome activation | ||
| NOD-like receptor family, pyrin-domain containing 1 (NLRP1) | Panx1 IP | (NLRP and/or cell-type dependent) | Silverman et al. (2009) |
| NOD-like receptor family, pyrin-domain containing 2 (NLRP2) | NLRP2 IP | Minkiewicz et al. (2013) | |
| NOD-like receptor family, pyrin-domain containing 2 (NLRP3) | FLAG and HA IPs | Wang et al. (2013) | |
| Apoptosis-associated speck-like protein containing CARD (ASC) | Panx1 and ASC IP ASC IP | Silverman et al. (2009), Minkiewicz et al. (2013) | |
| X-linked inhibitor of apoptosis (XIAP) | Panx1 IP | Silverman et al. (2009) | |
| Caspase 1 (Casp1) | Panx1 IP | Silverman et al. (2009) | |
| Caspase 11 (Casp11) | Panx1 IP | Silverman et al. (2009) | |
| Purinergic receptor, P2X, ligand-gated ion channel 4 (P2X4 receptor) | P2X4 IP | ATP-induced ROS production | Hung et al. (2013) |
| Voltage-gated potassium channel subunit beta-3 (Kcnab3) | B2H and co-loc HA and MYC IPs | Modulating Panx1 redox sensitivity and Panx1 inactivation | Bunse et al. (2005, 2009) |
| α-1D adrenergic receptor (Adra1d) | Panx1 and Adra1d IPs | Panx1 activation coupled to adrenoreceptor function | Billaud et al. (2011) |
| Possible role in blood pressure regulation | |||
| Cytoskeletal Proteins | |||
| Actin | Panx1 (OE) IP and BA | Panx1 trafficking and stabilization | Bhalla-Gehi et al. (2010), Wicki-Stordeur and Swayne (2013) |
| Panx1 IP and co-loc (OE) | |||
| Actin-related protein 3 (Arp3) | GFP IP and LC-MS/MS | Unknown | Wicki-Stordeur and Swayne (2013) |
| Panx1 IP and co-loc (OE) |
B2H, bacterial two-hybrid; BA, binding assay; Co-loc, Co-localization; Endg, endogenous; IP, immunoprecipitation; OE, overexpressed; LC-MS/MS, high performance liquid chromatography coupled to tandem mass spectrometry analysis.