Table 1. Melting temperatures of DNA triplexes and duplexes in 4 M NaCl and 4 M choline dhp^a.

	Tm(°C) at 260 nmb
Condition/Sequenceb	Tm-H	Tm-W	Tm-H&W	Tm-W
4 M NaCl
Ts1 (2)c			39.4	Ds1 (2)d	43.8
Ts2 (4)	14.5	48.1		Ds2 (4)	47.5
Ts3 (6)	n.d.e	51.6		Ds3 (6)	51.2
4 M choline dhp
Ts1 (2)c			55.5	Ds1 (2)d	51.4
Ts2 (4)			49.3	Ds2 (4)	43.2
Ts3 (6)			37.3	Ds3 (6)	40.2

^aAll experiments were conducted in a buffer containing 50 mM Tris (pH 7.0), 1 mM Na₂EDTA and 4 M NaCl or 4 M choline dhp.

^bTotal DNA strand concentration of triplexes and duplexes were 30 and 20 μM, respectively.

^cThe number of G*C Hoogsteen base pairs is shown in parentheses.

^dThe number of G–C Watson–Crick base pairs is shown in parentheses.

^en.d. indicates that the T_m-H was too low to be determined.