Table 2. Thermodynamic parameters for the formation of DNA triplexes and duplexes measured in 4 M NaCl or 4 M choline dhp^a.

	ΔH° (kcal mol−1)	TΔS° (kcal mol−1)	ΔG°25 (kcal mol−1)	Tmb (°C)
4 M NaCl
iTs1	−80.3 ± 8.7	−67.3 ± 7.7	−13.1 ± 2.5	78.5
iDs1	−57.2 ± 1.1	−48.4 ± 0.9	−8.8 ± 0.7	75.3
4 M Choline dhp
iTs1	−96.3 ± 8.7	−78.8 ± 7.7	−17.5 ± 2.5	83.9
iDs3	−57.4 ± 1.0	−48.5 ± 0.9	−8.9 ± 0.7	77.3

^aAll experiments were conducted in a buffer containing 50 mM Tris (pH 7.0), 1 mM Na₂EDTA and 4 M NaCl or 4 M choline dhp. Thermodynamic parameters were evaluated from curve fitting.

^bMelting temperature was calculated at a strand concentration of 30 μM.