FIGURE 6.
MgSO4 decreases IL-6 and TNF-α gene expression following LPS stimulation. CBMCs in the presence (Mg Sup) or absence (CTRL) of magnesium supplementation were stimulated with LPS; RNA was extracted and reverse transcribed at the time points shown. Analysis of real-time PCR, showing the relative abundance of mRNAs encoding for IL-6 and TNF-α normalized relative to a stably expressed housekeeping gene (Gus), is shown. To control for differences in RNA extraction and RT, PCR efficiency samples were run in triplicate; error bars (SEM) are shown. For IL-6 at 1, 2, and 4 h time points and TNF-α at the 2 and 4 h time points, p < 0.05. Data shown are representative of three individual experiments using different donors.