Skip to main content
. Author manuscript; available in PMC: 2014 Jan 8.
Published in final edited form as: J Immunol. 2012 May 18;188(12):10.4049/jimmunol.1101765. doi: 10.4049/jimmunol.1101765

FIGURE 9.

FIGURE 9

MgSO4 increases IκBα levels. (A) Neonatal monocytes were stimulated with 50 pg/ml LPS, and IκBα levels, normalized to tubulin, a stably expressed housekeeping protein, were assessed at the indicated time points. Numbers below the blot indicate the IκBα/tubulin ratio, as calculated via infrared imaging, representing normalized IκBα levels. (B) Cyclohexamide (CHX) treatment maintains increased IκBα levels. THP-1 cells were treated with CHX, and after 1 h magnesium was added for an additional 2 h in the absence of stimulation. Cell lysates were analyzed by Western blotting, and IκBα levels under each condition are shown. Results are representative of three experiments.