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RSK3 phosphorylates rpS6. (A) PaTu-8988t cells were transiently transfected with siRSK3 or siC at two different siRNA concentrations (25 and 50 nM). Phosphorylated rpS6 and total rpS6 were detected with specific antibodies. (B) PaTu-8988t cells were treated with the RSK inhibitor BI-D1870 (5 µM) and/or erlotinib (10 µM) for the indicated time points. Phospho-rpS6 and total rpS6 were detected by specific antibodies.
