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. 2013 Dec;15(12):1363–1370. doi: 10.1593/neo.11606

Figure 1.

Figure 1

N-Myc alters the balance of MXI1-0 and MXI1 mRNA expression. MXI1-0 and MXI1 expression in SHEP and SHEP/MYCN cells. Real-time RT-PCR was performed on RNA from SHEP and SHEP/MYCN cells using primers specific for MXI1-0 and MXI1. (A) An amplification plot was generated each for cell type and amplified transcript. The cycle number at threshold fluorescence was also determined. (B) Real-time PCR was also run on various concentrations of pcDNA3.1/MXI1-0 or pcDNA3.1/MXI1 to generate cycle number to copy number standard curves. The cycle numbers generated from the SHEP and SHEP/MYCN RNA were plotted on the standard curves to extrapolate copy numbers. Extrapolated copy numbers were plotted and compared. *P < .05 compared to SHEP MXI1. #P < .05 compared to SHEP MXI1-0.