(A and B) Small-intestinal (“SI”) and large-intestinal (“LI”) LPLs were isolated from Ahr−/− and littermate Ahr+/+ mice. RORγt expression inCD4+TCRβ+ cells was analyzed by flow cytometry, as shown in (A). Percentages of RORγt+ cells gated on CD4+TCRβ+ cells are shown in (B). Data are representative of two independent experiments. Horizontal lines show the mean. Error bars represent the SEM.
(C) Small-intestinal LPLs were isolated from Ahr−/− (n = 3) or Rorcgfp/gfpAhr−/− (n = 6) littermate mice. Cells were stimulated with PMA and ionomycin for 4 hr. Expression of IL-17 and IL-22 in CD4+TCRβ+ cells was analyzed by flow cytometry. Percentages of IL-17 and IL-22 gated on CD4+ TCRβ+ cells are shown. Data are representative of three independent experiments. Horizontal lines show the mean. Error bars represent the SEM.