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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Oct 3;273(2):10.1016/j.taap.2013.09.021. doi: 10.1016/j.taap.2013.09.021

Figure 5.

Figure 5

Regulation of β-catenin in human skin cancer cells. (A) siRNA knockdown of COX-2 in A431 and SCC13 cells resulted in decrease in levels of β-catenin and its downstream target c-Myc, as determined by western blot analysis. (B) siRNA knockdown of COX-2 in A431 or SCC13 cells resulted in significant reduction in cell viability. (C) siRNA knockdown of β-catenin in A431 and SCC13 cells resulted in lowering the levels of VEGF and c-Myc, as analyzed by western blot analysis. (D) siRNA knockdown of β-catenin in A431 and SCC13 cells resulted in significant decrease in cell viability. Briefly, for the analysis of cell viability, 5×104 cells were plated in six well culture plates. Twenty-four h later, cells were harvested, counted using a microscope and the cell numbers were compared between control siRNA and β-catenin or COX-2 siRNA knockdown groups of cells. Cells treated with scrambled siRNA were used as a control group. Significant inhibition vs control, *P<0.01.